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M9170
Sigma
Macrophage Colony-Stimulating Factor from mouse
recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested
| Synonym: | CSF-1, M-CSF |
| CAS Number: | 81627-83-0 |
| MDL number: | MFCD00166591 |
Description
| Analysis Note | The proliferative activity is tested by the dose-dependent stimulation of the murine monocytic cell line, M-NFS-60. |
| Physical form | Lyophilized from a sterile filtered buffered aqueous solution. |
| Biochem/physiol Actions | Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by a number of cells, including fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF can stimulate the proliferation of isolated macrophages, augment the production and release of cytokines and other inflammatory modulators from macrophages, enhance macrophage antibody-dependent cell-mediated cytotoxicity, prime and enhance macrophages in their ability to kill tumor cells and other microorganisms, stimulate pinocytosis, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing of a single M-CSF gene and due to variations in glycosylation, the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Human and mouse M-CSF share approximately 80% sequence homology in the conserved N-terminal region of 150 amino acids. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions. |
Properties
| recombinant | expressed in Escherichia coli |
| assay | ≥97% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | homodimer mol wt 18.2 kDa (containing 156 amino acid residues) |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| storage temp. | −20°C |
Safety
| Safety Statements | 22-24/25 |
| WGK Germany | 1 |
References
| reference | Stanley, E.R., Nicola, N., ed., Colony-stimulating factor-1. Guidebook to Cytokines and Their Receptors New York, N.Y. , (1994), 164-167 |
| Sherr, C., and Stanley, E.R,, Sporn, M., and Roberts, A, ed., Colony-stimulating factor-1 (macrophage colony-stimulating factor). Peptide Growth Factors and their Receptors I New York , (1991), 667-698 | |
| Callard, R., and Gearing, A. The Cytokine Facts Book New York , (1994) | |
| Rohrschneider, L.R., Nicola, N., ed., The macrophage colony-stimulating factor (M-CSF) receptor. Guidebook to Cytokines and Their Receptors New York , (1994), 168-170 | |
| Yarden, Y., and Ullrich, A., Growth factor receptor tyrosine kinases. Annu. Rev. Biochem. 57, 443-478, (1988) | |
| Heim, M., The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J. Recept. Signal Transduct. Res. 19, 75-120, (1999) |
